TST38

  Prothallia of Adiantum capillus-veneris were grown under white light of 4.8-5.8 W m^-2from fluorescent lamps (FL40SD; Toshiba Lighting and Technology) at 25°C. Total RNA was prepared as described by Kanegae and Wada (1998). To purify poly(A)+ RNA, we used an mRNA purification kit (Amersham Pharmacia Biotech) according to the manufacturer’s instructions as described by Yamauchi et al. (2005).  cDNA libraries were synthesized with CloneMiner cDNA library construction kit (Invitrogen).

YMU01

  Prothallia of Adiantum capillus-veneris were grown under white light of 4.8-5.8 W m^-2from fluorescent lamps (FL40SD; Toshiba Lighting and Technology) at 25°C. Total RNA was prepared from the prothallia as described by Kanegae and Wada (1998). To purify poly(A)+ RNA, we used an mRNA purification kit (Amersham Pharmacia Biotech) according to the manufacturer’s instructions.

Normalization of cDNA was carried out as described by Kohchi et al. (1995) with some modifications. The cDNA equalization cycle was repeated three times.  The cDNA obtained was electrophoresed on a 2% agarose gel with NuSieve GTG (FMC BioProduct) and cDNA ranging in size from 0.5-1 kb was purified using a Geneclean kit (Qbiogene), and cloned into the pCR4-TOPO vector (Invitrogen). Details are described in Yamauchi et al. (2005).