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NBDC - LSDB Archive
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DK959679
Clone id TST39A01NGRL0005_F16
Library
Length 676
Definition Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0005_F16. 5' end sequence.
Accession
Tissue type prothallia with plantlets
Developmental stage gametophytes with sporophytes
Contig ID -
Sequence 
ATACTGTCTTATTCTGAGAGAGAGAGAGAGAGAGAGAGACAGAGAGAGCTTCTTGTTGCC
AGTTCCAATTTTGCAGACTCTCAAAGGAGAGGCTGGAGGCTGGGAGGGTGATTGCTGAAC
ATCAAGCTCACCATGCCGGCGCATGGCCCAAAAGGGTAGCCCTGTACTTTAGTTTTCGTT
TAGGGAAGCAGCTGAGAGAGAATGATTCTCTCTGTAAAGAATGCAGAACTTTTACTTACA
TAGTGCAGCTCAGCGTTGAGTGCTAATAACAACTAGGCTCCGGCATTGGTACGCTCACAG
CCGAGTGAAGAGATGGATGAGCATGGGAGCCCTATGGCTCCAACATACATTTTCAGGTCG
AAGCTCCCTGAGATCGACATACCAAGTCATCTGTCCCTCACCCAATATTGCTTTCAACAT
ATTGCGAAATTTGCCCACAGGCCCTGCTTGGTTGAGAGCACTGGAAAGAATTACACTTTT
GCAGAGGTTGAGCTCTACACTCGGCGCGTTGCCGCAGGATTCTGTCGTTTGGGCATTGAG
AGAGGGGATGTTGTTATGCTCTTACTGCCCAACTGTGTGGAATTTGTACTCCTCTTCCTG
GGGCTTGCAATGAGAGGAGCTGCTGCCACTACTTGTAATCCTTCCTACACCGCCCAGGAG
ATCTCAAAGCAGGTGA
■■Homology search results ■■ -
sp_hit_id P41636
Definition sp|P41636|4CL_PINTA 4-coumarate--CoA ligase OS=Pinus taeda
Align length 110
Score (bit) 134.0
E-value 6.0e-31
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959679|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0005_F16, 5'
         (676 letters)

Database: uniprot_sprot.fasta 
           412,525 sequences; 148,809,765 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P41636|4CL_PINTA 4-coumarate--CoA ligase OS=Pinus taeda GN=4C...   134   6e-31
sp|Q9S777|4CL3_ARATH 4-coumarate--CoA ligase 3 OS=Arabidopsis th...   130   5e-30
sp|Q42524|4CL1_ARATH 4-coumarate--CoA ligase 1 OS=Arabidopsis th...   128   2e-29
sp|Q67W82|4CL4_ORYSJ Probable 4-coumarate--CoA ligase 4 OS=Oryza...   126   9e-29
sp|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 OS=Glycine max PE...   124   6e-28
sp|Q6ETN3|4CL3_ORYSJ Probable 4-coumarate--CoA ligase 3 OS=Oryza...   120   5e-27
sp|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 OS=Solanum tubero...   119   1e-26
sp|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 OS=Solanum tubero...   119   1e-26
sp|Q42982|4CL2_ORYSJ Probable 4-coumarate--CoA ligase 2 OS=Oryza...   119   2e-26
sp|O24540|4CL_VANPL 4-coumarate--CoA ligase OS=Vanilla planifoli...   118   3e-26
sp|P14913|4CL2_PETCR 4-coumarate--CoA ligase 1 OS=Petroselinum c...   118   3e-26
sp|P14912|4CL1_PETCR 4-coumarate--CoA ligase 1 OS=Petroselinum c...   118   3e-26
sp|O24146|4CL2_TOBAC 4-coumarate--CoA ligase 2 OS=Nicotiana taba...   116   1e-25
sp|O24145|4CL1_TOBAC 4-coumarate--CoA ligase 1 OS=Nicotiana taba...   115   2e-25
sp|Q9LU36|4CL4_ARATH 4-coumarate--CoA ligase 4 OS=Arabidopsis th...   112   2e-24
sp|Q9S725|4CL2_ARATH 4-coumarate--CoA ligase 2 OS=Arabidopsis th...   112   2e-24
sp|Q6ZAC1|4CL5_ORYSJ Probable 4-coumarate--CoA ligase 5 OS=Oryza...   106   1e-22
sp|P17814|4CL1_ORYSJ Probable 4-coumarate--CoA ligase 1 OS=Oryza...    96   2e-19
sp|Q9M0X9|4CLL7_ARATH 4-coumarate--CoA ligase-like 7 OS=Arabidop...    78   4e-14
sp|Q9LQ12|4CLL1_ARATH 4-coumarate--CoA ligase-like 1 OS=Arabidop...    78   5e-14
sp|Q7XXL2|4CLL9_ORYSJ 4-coumarate--CoA ligase-like 9 OS=Oryza sa...    71   5e-12
sp|Q84P24|4CLL6_ARATH 4-coumarate--CoA ligase-like 6 OS=Arabidop...    69   3e-11
sp|Q84P21|4CLL5_ARATH 4-coumarate--CoA ligase-like 5 OS=Arabidop...    64   1e-09
sp|Q84P25|4CLL2_ARATH 4-coumarate--CoA ligase-like 2 OS=Arabidop...    62   2e-09
sp|Q336M7|4CLL2_ORYSJ 4-coumarate--CoA ligase-like 2 OS=Oryza sa...    62   4e-09
sp|Q0DV32|4CLL1_ORYSJ 4-coumarate--CoA ligase-like 1 OS=Oryza sa...    62   4e-09
sp|Q54P77|4CL1_DICDI Probable 4-coumarate--CoA ligase 1 OS=Dicty...    60   8e-09
sp|Q54P78|4CL2_DICDI Probable 4-coumarate--CoA ligase 2 OS=Dicty...    59   2e-08
sp|Q54P79|4CL3_DICDI Probable 4-coumarate--CoA ligase 3 OS=Dicty...    58   4e-08
sp|Q6YYZ2|4CLL3_ORYSJ 4-coumarate--CoA ligase-like 3 OS=Oryza sa...    56   2e-07

>sp|P41636|4CL_PINTA 4-coumarate--CoA ligase OS=Pinus taeda GN=4CL
           PE=2 SV=1
          Length = 537

 Score =  134 bits (336), Expect = 6e-31
 Identities = 60/110 (54%), Positives = 85/110 (77%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGF 522
           +++RSKLP+I+I  HL L  YCF+ +A+FA RPCL++ +T + Y F+EVEL +R+VAAG 
Sbjct: 10  HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGL 69

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
            +LG+++G VVMLLLPNC+EF  +F+G ++RGA  TT NP Y   EI+KQ
Sbjct: 70  AKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQ 119


>sp|Q9S777|4CL3_ARATH 4-coumarate--CoA ligase 3 OS=Arabidopsis
           thaliana GN=4CL3 PE=1 SV=1
          Length = 561

 Score =  130 bits (328), Expect = 5e-30
 Identities = 65/117 (55%), Positives = 85/117 (72%), Gaps = 1/117 (0%)
 Frame = +1

Query: 328 SPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCL-VESTGKNYTFAEVELYTR 504
           SP  P  IFRSKLP+IDIP+HL L  YCF+ ++  + +PCL V STGK+YT+ E  L  R
Sbjct: 28  SPPTPR-IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICR 86

Query: 505 RVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQV 675
           RVA+G  +LGI +GDV+M+LL N  EFV  F+G +M GA +TT NP YT+QE+ KQ+
Sbjct: 87  RVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQL 143


>sp|Q42524|4CL1_ARATH 4-coumarate--CoA ligase 1 OS=Arabidopsis
           thaliana GN=4CL1 PE=1 SV=1
          Length = 561

 Score =  128 bits (322), Expect = 2e-29
 Identities = 64/124 (51%), Positives = 88/124 (70%), Gaps = 1/124 (0%)
 Frame = +1

Query: 304 SEEMDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTF 480
           S+ M++  +      IFRSKLP+I IP+HLSL  Y FQ+I++FA +PCL+   TG  YT+
Sbjct: 9   SQVMEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTY 68

Query: 481 AEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQE 660
           ++V + +R++AA F +LG+ + DVVMLLLPNC EFVL FL  + RGA AT  NP +T  E
Sbjct: 69  SDVHVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAE 128

Query: 661 ISKQ 672
           I+KQ
Sbjct: 129 IAKQ 132


>sp|Q67W82|4CL4_ORYSJ Probable 4-coumarate--CoA ligase 4 OS=Oryza
           sativa subsp. japonica GN=4CL4 PE=2 SV=1
          Length = 559

 Score =  126 bits (317), Expect = 9e-29
 Identities = 63/111 (56%), Positives = 78/111 (70%), Gaps = 1/111 (0%)
 Frame = +1

Query: 343 TYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTFAEVELYTRRVAAG 519
           T +FRSKLP+I+IPSHL+L  YCF+ + + A RPCL++  TG  Y++ EVE  +RR AAG
Sbjct: 16  TVVFRSKLPDIEIPSHLTLQAYCFEKLPEVAARPCLIDGQTGAVYSYGEVEELSRRAAAG 75

Query: 520 FCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
             RLG+ +GDVVM LL NC EF   FLG A  GAA TT NP YT  EI +Q
Sbjct: 76  LRRLGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEIHRQ 126


>sp|P31687|4CL2_SOYBN 4-coumarate--CoA ligase 2 OS=Glycine max PE=2
           SV=2
          Length = 562

 Score =  124 bits (310), Expect = 6e-28
 Identities = 60/125 (48%), Positives = 85/125 (68%), Gaps = 1/125 (0%)
 Frame = +1

Query: 301 PSEEMDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYT 477
           P  + ++   P   +++F+SKLP+I I +HL L  YCFQ++++FAHRPCL+     K +T
Sbjct: 11  PKTDQNQVSDPQT-SHVFKSKLPDIPISNHLPLHSYCFQNLSQFAHRPCLIVGPASKTFT 69

Query: 478 FAEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQ 657
           +A+  L + ++AAG   LGI +GDVVM+LL N  +FV  FL ++M GA ATT NP YTA 
Sbjct: 70  YADTHLISSKIAAGLSNLGILKGDVVMILLQNSADFVFSFLAISMIGAVATTANPFYTAP 129

Query: 658 EISKQ 672
           EI KQ
Sbjct: 130 EIFKQ 134


>sp|Q6ETN3|4CL3_ORYSJ Probable 4-coumarate--CoA ligase 3 OS=Oryza
           sativa subsp. japonica GN=4CL3 PE=2 SV=1
          Length = 554

 Score =  120 bits (302), Expect = 5e-27
 Identities = 58/109 (53%), Positives = 78/109 (71%), Gaps = 1/109 (0%)
 Frame = +1

Query: 349 IFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTFAEVELYTRRVAAGFC 525
           +FRSKLP+I+I + ++L +YCF  +A+   RPCL++  TG++YT+AEVE  +RR AAG  
Sbjct: 11  VFRSKLPDIEIDNSMTLQEYCFARMAEVGARPCLIDGQTGESYTYAEVESASRRAAAGLR 70

Query: 526 RLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
           R+G+ +GDVVM LL NC EF   FLG A  GAA TT NP YT  E+ +Q
Sbjct: 71  RMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQ 119


>sp|P31685|4CL2_SOLTU 4-coumarate--CoA ligase 2 OS=Solanum tuberosum
           GN=4CL2 PE=3 SV=1
          Length = 545

 Score =  119 bits (298), Expect = 1e-26
 Identities = 56/109 (51%), Positives = 77/109 (70%), Gaps = 1/109 (0%)
 Frame = +1

Query: 349 IFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVESTGKN-YTFAEVELYTRRVAAGFC 525
           IFRSKLP+I IP HL L  YCF+++++F  RPCL++      YT+AEVEL +R+VA G  
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 526 RLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
           +LGI++ D +M+LLPNC EFV  F+G +  GA +T  NP +T  E+ KQ
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122


>sp|P31684|4CL1_SOLTU 4-coumarate--CoA ligase 1 OS=Solanum tuberosum
           GN=4CL1 PE=3 SV=1
          Length = 545

 Score =  119 bits (298), Expect = 1e-26
 Identities = 56/109 (51%), Positives = 77/109 (70%), Gaps = 1/109 (0%)
 Frame = +1

Query: 349 IFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVESTGKN-YTFAEVELYTRRVAAGFC 525
           IFRSKLP+I IP HL L  YCF+++++F  RPCL++      YT+AEVEL +R+VA G  
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 526 RLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
           +LGI++ D +M+LLPNC EFV  F+G +  GA +T  NP +T  E+ KQ
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122


>sp|Q42982|4CL2_ORYSJ Probable 4-coumarate--CoA ligase 2 OS=Oryza
           sativa subsp. japonica GN=4CL2 PE=2 SV=2
          Length = 569

 Score =  119 bits (297), Expect = 2e-26
 Identities = 62/127 (48%), Positives = 75/127 (59%), Gaps = 1/127 (0%)
 Frame = +1

Query: 295 SQPSEEMDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKN 471
           +QP        +P     +FRSKLP+IDIPSHL L +YCF   A+    PCL+ + TG+ 
Sbjct: 9   AQPQVAAAVDEAPPEAVTVFRSKLPDIDIPSHLPLHEYCFARAAELPDAPCLIAAATGRT 68

Query: 472 YTFAEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYT 651
           YTFAE  L  RR AA   RLG+  GD VM+LL NCVEF + F   +  GA  T  NP  T
Sbjct: 69  YTFAETRLLCRRAAAALHRLGVGHGDRVMVLLQNCVEFAVAFFAASFLGAVTTAANPFCT 128

Query: 652 AQEISKQ 672
            QEI KQ
Sbjct: 129 PQEIHKQ 135


>sp|O24540|4CL_VANPL 4-coumarate--CoA ligase OS=Vanilla planifolia
           GN=4CL PE=3 SV=1
          Length = 553

 Score =  118 bits (296), Expect = 3e-26
 Identities = 56/109 (51%), Positives = 81/109 (74%), Gaps = 1/109 (0%)
 Frame = +1

Query: 349 IFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGFC 525
           IFRSKLP+I IP +L L  YCF++I+KF+ RPCL+  +T + +T+A+VEL +RRV +G  
Sbjct: 15  IFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGSGLS 74

Query: 526 RLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
           +LGI++GD +M+LLPN  EFV  FLG +  G+ +T  NP +T+ E+ KQ
Sbjct: 75  KLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 123
tr_hit_id Q3YAU2
Definition tr|Q3YAU2|Q3YAU2_EUCCA 4-coumarate:coenzyme A ligase OS=Eucalyptus camaldulensis
Align length 129
Score (bit) 136.0
E-value 1.0e-30
Report 
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DK959679|Adiantum capillus-veneris mRNA, clone:
TST39A01NGRL0005_F16, 5'
         (676 letters)

Database: uniprot_trembl.fasta 
           7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

tr|Q3YAU2|Q3YAU2_EUCCA 4-coumarate:coenzyme A ligase OS=Eucalypt...   136   1e-30
tr|A8R7L9|A8R7L9_EUCGG 4-coumarate:coenzyme A ligase OS=Eucalypt...   135   2e-30
tr|B8LQR8|B8LQR8_PICSI Putative uncharacterized protein OS=Picea...   134   4e-30
tr|B8QN50|B8QN50_PINRA 4-coumarate-CoA ligase OS=Pinus radiata P...   134   7e-30
tr|A7Q6G3|A7Q6G3_VITVI Chromosome chr11 scaffold_56, whole genom...   134   7e-30
tr|B1GUX0|B1GUX0_ARALY 4-cumarate-COA-ligase (Fragment) OS=Arabi...   132   3e-29
tr|B7SBA8|B7SBA8_PHYPA 4-coumarate:coenzyme A ligase 2 OS=Physco...   131   3e-29
tr|B3TNC2|B3TNC2_9POAL 4-coumarate:CoA ligase OS=Neosinocalamus ...   131   3e-29
tr|Q9LL50|Q9LL50_RUBID 4-coumarate:coA ligase 2 OS=Rubus idaeus ...   131   4e-29
tr|B7SBB0|B7SBB0_PHYPA 4-coumarate:coenzyme A ligase 4 OS=Physco...   131   4e-29
tr|A5AXP2|A5AXP2_VITVI Putative uncharacterized protein OS=Vitis...   131   4e-29
tr|Q3ECI2|Q3ECI2_ARATH Uncharacterized protein At1g65060.2 OS=Ar...   130   6e-29
tr|B7SBA7|B7SBA7_PHYPA 4-coumarate:coenzyme A ligase 1 OS=Physco...   130   6e-29
tr|B7SB99|B7SB99_PHYPA 4-coumarate:coenzyme A ligase 1 OS=Physco...   130   6e-29
tr|A9T8V9|A9T8V9_PHYPA Predicted protein OS=Physcomitrella paten...   130   6e-29
tr|B7SBA5|B7SBA5_PHYPA 4-coumarate:coenzyme A ligase 3 OS=Physco...   130   1e-28
tr|B7SBA2|B7SBA2_PHYPA 4-coumarate:coenzyme A ligase 4 OS=Physco...   130   1e-28
tr|Q94G18|Q94G18_POPJC 4-coumarate:CoA ligase 4 OS=Populus jacki...   129   1e-28
tr|B7SBA4|B7SBA4_PHYPA 4-coumarate:coenzyme A ligase 2 OS=Physco...   129   1e-28
tr|B7SBA1|B7SBA1_PHYPA 4-coumarate:coenzyme A ligase 3 OS=Physco...   129   1e-28
tr|A9T5A3|A9T5A3_PHYPA Predicted protein OS=Physcomitrella paten...   129   1e-28
tr|A9RP51|A9RP51_PHYPA Predicted protein OS=Physcomitrella paten...   129   1e-28
tr|A9PGE3|A9PGE3_POPTR Putative uncharacterized protein OS=Popul...   129   1e-28
tr|B7SBA9|B7SBA9_PHYPA 4-coumarate:coenzyme A ligase 3 OS=Physco...   129   2e-28
tr|B7SBA6|B7SBA6_PHYPA 4-coumarate:coenzyme A ligase 4 OS=Physco...   129   2e-28
tr|B7SBA0|B7SBA0_PHYPA 4-coumarate:coenzyme A ligase 2 OS=Physco...   129   2e-28
tr|B1GUW8|B1GUW8_ARATH 4-cumarate-COA-ligase (Fragment) OS=Arabi...   128   3e-28
tr|B1GUW0|B1GUW0_ARATH 4-cumarate-COA-ligase (Fragment) OS=Arabi...   128   3e-28
tr|B1GUV7|B1GUV7_ARATH 4-cumarate-COA-ligase (Fragment) OS=Arabi...   128   3e-28
tr|B1GUV5|B1GUV5_ARATH 4-cumarate-COA-ligase (Fragment) OS=Arabi...   128   3e-28

>tr|Q3YAU2|Q3YAU2_EUCCA 4-coumarate:coenzyme A ligase OS=Eucalyptus
           camaldulensis GN=4CL1 PE=2 SV=1
          Length = 544

 Score =  136 bits (343), Expect = 1e-30
 Identities = 70/129 (54%), Positives = 94/129 (72%), Gaps = 1/129 (0%)
 Frame = +1

Query: 289 VRSQPSEEMDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STG 465
           + ++PSE+  E        +IFRSKLP+I IP +LSL  YCF++I++FA RPC++  +TG
Sbjct: 1   MEAKPSEQPRE--------FIFRSKLPDIYIPDNLSLHAYCFENISEFADRPCVINGATG 52

Query: 466 KNYTFAEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPS 645
           + YT+AEVEL +RRV+AG   LG+ +GDV+MLLL NC EFV  FLG + RGA +TT NP 
Sbjct: 53  RTYTYAEVELISRRVSAGLNGLGVGQGDVIMLLLQNCPEFVFAFLGASYRGAISTTANPF 112

Query: 646 YTAQEISKQ 672
           YT  EI+KQ
Sbjct: 113 YTPGEIAKQ 121


>tr|A8R7L9|A8R7L9_EUCGG 4-coumarate:coenzyme A ligase OS=Eucalyptus
           globulus subsp. globulus GN=Egl4CL PE=3 SV=2
          Length = 544

 Score =  135 bits (341), Expect = 2e-30
 Identities = 70/129 (54%), Positives = 94/129 (72%), Gaps = 1/129 (0%)
 Frame = +1

Query: 289 VRSQPSEEMDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STG 465
           + ++PSE+  E        +IFRSKLP+I IP +LSL  YCF++I++FA RPC++  +TG
Sbjct: 1   MEAKPSEQPRE--------FIFRSKLPDIYIPDNLSLHAYCFENISEFADRPCVINGATG 52

Query: 466 KNYTFAEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPS 645
           + YT+AEVEL +RRV+AG   LG+ +GDV+MLLL NC EFV  FLG + RGA +TT NP 
Sbjct: 53  QTYTYAEVELISRRVSAGLNGLGVGQGDVIMLLLRNCPEFVFAFLGASYRGAISTTANPF 112

Query: 646 YTAQEISKQ 672
           YT  EI+KQ
Sbjct: 113 YTPGEIAKQ 121


>tr|B8LQR8|B8LQR8_PICSI Putative uncharacterized protein OS=Picea
           sitchensis PE=2 SV=1
          Length = 537

 Score =  134 bits (338), Expect = 4e-30
 Identities = 62/110 (56%), Positives = 84/110 (76%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGF 522
           +++RSKLP+I+I  HLSL  YCF+ +A+FA RPCL++ +T K Y FAEVEL +R+VAAG 
Sbjct: 10  HLYRSKLPDIEISDHLSLHSYCFERVAEFADRPCLIDGATDKTYCFAEVELISRKVAAGL 69

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
             LG+++G VVMLLL NC+EF  +F+G ++RGA  TT NP Y   EI+KQ
Sbjct: 70  ANLGLQQGQVVMLLLQNCIEFAFVFMGASVRGAIVTTANPFYKPAEIAKQ 119


>tr|B8QN50|B8QN50_PINRA 4-coumarate-CoA ligase OS=Pinus radiata PE=2
           SV=1
          Length = 537

 Score =  134 bits (336), Expect = 7e-30
 Identities = 60/110 (54%), Positives = 85/110 (77%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGF 522
           +++RSKLP+I+I  HL L  YCF+ +A+FA RPCL++ +T + Y F+EVEL +R+VAAG 
Sbjct: 10  HLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGL 69

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
            +LG+++G VVMLLLPNC+EF  +F+G ++RGA  TT NP Y   EI+KQ
Sbjct: 70  AKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQ 119


>tr|A7Q6G3|A7Q6G3_VITVI Chromosome chr11 scaffold_56, whole genome
           shotgun sequence OS=Vitis vinifera GN=GSVIVT00031385001
           PE=3 SV=1
          Length = 539

 Score =  134 bits (336), Expect = 7e-30
 Identities = 65/110 (59%), Positives = 80/110 (72%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTFAEVELYTRRVAAGF 522
           +I+RSKLP+I+IPSHL L  YCFQHI+KF+  PCL+ +  G  YT+A+V L  R+VAAG 
Sbjct: 7   FIYRSKLPDINIPSHLPLHSYCFQHISKFSSNPCLINAGNGNIYTYADVHLTARKVAAGL 66

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
            RLGI +GD +MLLL NC EFV  FLG +  GA +TT NP YT  EI KQ
Sbjct: 67  NRLGIRQGDAIMLLLQNCPEFVFSFLGASYLGATSTTANPFYTPAEIEKQ 116


>tr|B1GUX0|B1GUX0_ARALY 4-cumarate-COA-ligase (Fragment)
           OS=Arabidopsis lyrata GN=4CL1 PE=3 SV=1
          Length = 552

 Score =  132 bits (331), Expect = 3e-29
 Identities = 68/126 (53%), Positives = 89/126 (70%), Gaps = 2/126 (1%)
 Frame = +1

Query: 301 PSEE-MDEHGSPMAPTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNY 474
           P EE M++  +      IFRSKLP+I IP+HLSL  Y FQ+I++FA +PCL+   TG  Y
Sbjct: 3   PQEEVMEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVY 62

Query: 475 TFAEVELYTRRVAAGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTA 654
           T+++V + +RR+AAGF +LG+   DVVMLLLPNC EFVL FL  + RGA AT  NP +T 
Sbjct: 63  TYSDVHVISRRIAAGFHKLGVNHNDVVMLLLPNCPEFVLSFLAASFRGAIATAANPFFTP 122

Query: 655 QEISKQ 672
            EI+KQ
Sbjct: 123 AEIAKQ 128


>tr|B7SBA8|B7SBA8_PHYPA 4-coumarate:coenzyme A ligase 2
           OS=Physcomitrella patens subsp. magdalenae GN=4CL2 PE=4
           SV=1
          Length = 583

 Score =  131 bits (330), Expect = 3e-29
 Identities = 58/110 (52%), Positives = 84/110 (76%), Gaps = 1/110 (0%)
 Frame = +1

Query: 349 IFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGFC 525
           I+RSKLP+IDIP+H+ L  YCF+  A++  + CL++ +TG+ +T+ E+E+  RRVAAG  
Sbjct: 62  IYRSKLPDIDIPNHMPLADYCFEKAAQWPEKVCLIDGNTGRKHTYGEIEVSMRRVAAGLA 121

Query: 526 RLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQV 675
           ++G+++GDV+ LLLPNC EFV +FLG A RGA  TT NP YT+ E+ KQ+
Sbjct: 122 KIGVKQGDVIALLLPNCAEFVQVFLGAAKRGAVITTANPFYTSAELRKQI 171


>tr|B3TNC2|B3TNC2_9POAL 4-coumarate:CoA ligase OS=Neosinocalamus
           affinis PE=2 SV=1
          Length = 557

 Score =  131 bits (330), Expect = 3e-29
 Identities = 65/113 (57%), Positives = 81/113 (71%), Gaps = 1/113 (0%)
 Frame = +1

Query: 337 APTYIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTFAEVELYTRRVA 513
           AP  +FRSKLP+I+IPSHL+L  YCF+ + + A RPCL++  +G  YT+AEVE  +RR A
Sbjct: 11  APELVFRSKLPDIEIPSHLTLQAYCFERLPEVASRPCLIDGQSGAVYTYAEVEELSRRAA 70

Query: 514 AGFCRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
           AG  RLG+ +GDVVM LL NC EF   FLG A+ GAA TT NP YT  EI +Q
Sbjct: 71  AGLRRLGVGKGDVVMNLLRNCPEFAFTFLGAALLGAATTTANPFYTPHEIHRQ 123


>tr|Q9LL50|Q9LL50_RUBID 4-coumarate:coA ligase 2 OS=Rubus idaeus
           PE=2 SV=1
          Length = 544

 Score =  131 bits (329), Expect = 4e-29
 Identities = 64/110 (58%), Positives = 84/110 (76%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVE-STGKNYTFAEVELYTRRVAAGF 522
           +IFRSKLP+I IP+HL L  YCF++I++F  RPCL+  +TG+ +T+AEVEL +RRVAAG 
Sbjct: 11  FIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELTSRRVAAGL 70

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
            +LGI++ DVVMLLL NC EF   FLG +  GA +TT NP YT  E++KQ
Sbjct: 71  DKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQ 120


>tr|B7SBB0|B7SBB0_PHYPA 4-coumarate:coenzyme A ligase 4
           OS=Physcomitrella patens subsp. magdalenae GN=4CL4 PE=4
           SV=1
          Length = 570

 Score =  131 bits (329), Expect = 4e-29
 Identities = 59/110 (53%), Positives = 83/110 (75%), Gaps = 1/110 (0%)
 Frame = +1

Query: 346 YIFRSKLPEIDIPSHLSLTQYCFQHIAKFAHRPCLVES-TGKNYTFAEVELYTRRVAAGF 522
           +I+RSKLP+IDIP+H+ L+ YC +  A++  + CL++  TG+ YT+ E+EL TRRVAAG 
Sbjct: 48  FIYRSKLPDIDIPNHMPLSDYCLEKAAQWPDKICLIDGVTGREYTYGEIELSTRRVAAGL 107

Query: 523 CRLGIERGDVVMLLLPNCVEFVLLFLGLAMRGAAATTCNPSYTAQEISKQ 672
            ++G+++GDV+ LLLPNC EFV +FLG A  GA  TT NP YT+ E+ KQ
Sbjct: 108 FKIGVKQGDVIALLLPNCAEFVQVFLGAAKMGAIVTTANPFYTSAELEKQ 157