DK950147
Clone id TST38A01NGRL0007_O05
Library
Length 706
Definition Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0007_O05. 5' end sequence.
Accession
Tissue type prothallia
Developmental stage gametophyte
Contig ID
Sequence
ATACGCAACACGGTTCTCTCTCTCACACACACAGCCGCATAGTACATACATTCCTTTCGC
TGCGCAGTGCGCGCGAGCCGCACCAAAAGAGCGCCGCACACTCAACAAAAGCGCCCACAT
CCTACAATACCTCTGCTGTGAATGATTGGTGAAGCGCCCAACGTCAGCCTTTCTCCGCAT
AAAACGTGTCAGGCTCAGGAGAGAACACGTATGAGCAGGGAACAATGATGAGCAGGAACA
TTCTAGCGGAGAAGGCCCTGCTTTTGGGCTCTGTAGAGCAGCGGGAGAAGTCGTCTTGGT
TTCTCCAATAGCCAGCCATGGACTCTGACACAGCCAAGCAACAATTTGGTGTGGGGAGAT
GAATGGCAGCAAAATTTTTTATTCTGGAGGGTGTTGCTCTTCTCCAGTTTCAGAAGAATC
TACTCTGGATTGTGAAATCTCCCTTTGCACTGGTGGTCGAAACCACCAACCGATCATTGA
GTGTGCACCATCACCATCATCAGCAGCACTCACAAATCAATGCTCAGAAAGTAGTGCAGC
GTATTTTCTATGGCCTCCTGTTTATCGCCCTCATGGCAGCGCAGGAGAACGAGCAAATTA
TTTCCAAGGTCTTCAAAAGGCTGTGGAGTTTGGATCGATTGAGAGGATCTTAAAAGGTCC
GCAAGCATCAACGCTTCTGGACCTCATGACCATACGAGCATACAAT
■■Homology search results ■■ -
sp_hit_id Q54PA8
Definition sp|Q54PA8|Y2080_DICDI Uncharacterized protein DDB_G0284671 OS=Dictyostelium discoideum
Align length 64
Score (bit) 32.7
E-value 2.0
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK950147|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0007_O05, 5'
(706 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q54PA8|Y2080_DICDI Uncharacterized protein DDB_G0284671 OS=Di... 33 2.0
sp|P11976|PSTA_DICDI Prestalk protein OS=Dictyostelium discoideu... 32 2.6
sp|Q5M951|CA174_RAT UPF0688 protein C1orf174 homolog OS=Rattus n... 32 4.4
sp|Q9D219|BCL9_MOUSE B-cell CLL/lymphoma 9 protein OS=Mus muscul... 30 9.8
sp|O00512|BCL9_HUMAN B-cell CLL/lymphoma 9 protein OS=Homo sapie... 30 9.8

>sp|Q54PA8|Y2080_DICDI Uncharacterized protein DDB_G0284671
OS=Dictyostelium discoideum GN=DDB_G0284671 PE=4 SV=1
Length = 305

Score = 32.7 bits (73), Expect = 2.0
Identities = 16/64 (25%), Positives = 30/64 (46%)
Frame = +2

Query: 395 CSSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAALTNQCSESSAAYFLWPPVYRPH 574
C+S + E T+ C++ CT + I C P+P++ + T+ + + A P
Sbjct: 48 CNSIIQEVYTIPCDLQTCTCKCTNGTIFGCQPTPTTTSTTSASTTTGKASSSGPSTTGRA 107

Query: 575 GSAG 586
S+G
Sbjct: 108 SSSG 111


>sp|P11976|PSTA_DICDI Prestalk protein OS=Dictyostelium discoideum
GN=ecmB PE=2 SV=2
Length = 1053

Score = 32.3 bits (72), Expect = 2.6
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 SGGCCSSPVSEESTLDCEISLCT--GGRNHQPIIECAPSPSSAALTNQCSESS 535
S GCC +P++ + C CT GG H PI A +A + CS S+
Sbjct: 287 STGCCHTPINVDDNNACTEDKCTQSGGVTHTPI---ACDDKNACTVDSCSNST 336



Score = 32.0 bits (71), Expect = 3.4
Identities = 17/53 (32%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 SGGCCSSPVSEESTLDCEISLCT--GGRNHQPIIECAPSPSSAALTNQCSESS 535
S GCC +P++ + C CT GG H PI + ++A + CS S+
Sbjct: 887 STGCCHTPINVDDNNPCTEDKCTQSGGVTHTPI---GCNDNNACTVDSCSNST 936



Score = 31.6 bits (70), Expect = 4.4
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 SGGCCSSPVSEESTLDCEISLCT--GGRNHQPIIECAPSPSSAALTNQCSESS 535
S GCC +P++ + C CT GG H PI A +A + CS S+
Sbjct: 143 STGCCHTPINVDDHNACTEDKCTQSGGVTHTPI---ACDDKNACTVDSCSNST 192



Score = 31.6 bits (70), Expect = 4.4
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2

Query: 383 SGGCCSSPVSEESTLDCEISLCT--GGRNHQPIIECAPSPSSAALTNQCSESS 535
S GCC +P++ + C CT GG H PI A +A + CS S+
Sbjct: 647 STGCCHTPINVDDHNACTEDKCTQSGGVTHTPI---ACDDKNACTVDSCSNST 696


>sp|Q5M951|CA174_RAT UPF0688 protein C1orf174 homolog OS=Rattus
norvegicus PE=2 SV=1
Length = 222

Score = 31.6 bits (70), Expect = 4.4
Identities = 29/103 (28%), Positives = 45/103 (43%)
Frame = +2

Query: 395 CSSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAALTNQCSESSAAYFLWPPVYRPH 574
CS PVSEE ++ E L T +P SAA + +SSA+ PV +
Sbjct: 117 CSKPVSEEPSVKAEEGLPT-------------APRSAAAAQETHDSSASQAEPVPVLQMD 163

Query: 575 GSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAY 703
S + Q + + FG++E + P AS+ M+ R +
Sbjct: 164 SSVFLDDDSNQPMPVSRFFGNVELMQDLPPASSSCPSMSRREF 206


>sp|Q9D219|BCL9_MOUSE B-cell CLL/lymphoma 9 protein OS=Mus musculus
GN=Bcl9 PE=1 SV=3
Length = 1425

Score = 30.4 bits (67), Expect = 9.8
Identities = 24/72 (33%), Positives = 36/72 (50%), Gaps = 3/72 (4%)
Frame = -2

Query: 456 PPVQREISQSRVDSSETGEEQHPPE*KILLPFISP--HQIVAWLCQSPWLAIGETKT-TS 286
PPVQR + + +D S G + H P + P SP HQ+ + + SP G K+ +
Sbjct: 834 PPVQRGLGRKPLDISVAGSQVHSPG---INPLKSPTMHQVQSPMLGSP---SGNLKSPQT 887

Query: 285 PAALQSPKAGPS 250
P+ L AGP+
Sbjct: 888 PSQLAGMLAGPA 899


>sp|O00512|BCL9_HUMAN B-cell CLL/lymphoma 9 protein OS=Homo sapiens
GN=BCL9 PE=1 SV=4
Length = 1426

Score = 30.4 bits (67), Expect = 9.8
Identities = 24/72 (33%), Positives = 36/72 (50%), Gaps = 3/72 (4%)
Frame = -2

Query: 456 PPVQREISQSRVDSSETGEEQHPPE*KILLPFISP--HQIVAWLCQSPWLAIGETKT-TS 286
PPVQR + + +D S G + H P + P SP HQ+ + + SP G K+ +
Sbjct: 835 PPVQRGLGRKPLDISVAGSQVHSPG---INPLKSPTMHQVQSPMLGSP---SGNLKSPQT 888

Query: 285 PAALQSPKAGPS 250
P+ L AGP+
Sbjct: 889 PSQLAGMLAGPA 900


tr_hit_id B8LKZ8
Definition tr|B8LKZ8|B8LKZ8_PICSI Putative uncharacterized protein OS=Picea sitchensis
Align length 109
Score (bit) 81.3
E-value 6.0e-14
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK950147|Adiantum capillus-veneris mRNA, clone:
TST38A01NGRL0007_O05, 5'
(706 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|B8LKZ8|B8LKZ8_PICSI Putative uncharacterized protein OS=Picea... 81 6e-14
tr|Q6Z9X3|Q6Z9X3_ORYSJ Os08g0407200 protein OS=Oryza sativa subs... 78 5e-13
tr|A7P2T6|A7P2T6_VITVI Chromosome chr1 scaffold_5, whole genome ... 78 5e-13
tr|A5BPH0|A5BPH0_VITVI Putative uncharacterized protein OS=Vitis... 78 5e-13
tr|A2YV45|A2YV45_ORYSI Putative uncharacterized protein OS=Oryza... 78 5e-13
tr|A7PWT6|A7PWT6_VITVI Chromosome chr19 scaffold_35, whole genom... 73 2e-11
tr|A2Q289|A2Q289_MEDTR Peptidase, trypsin-like serine and cystei... 71 8e-11
tr|A2Q138|A2Q138_MEDTR Peptidase, trypsin-like serine and cystei... 71 8e-11
tr|Q93YT5|Q93YT5_ARATH Putative uncharacterized protein At2g3515... 69 2e-10
tr|Q8S8Q2|Q8S8Q2_ARATH Expressed protein (Putative uncharacteriz... 69 2e-10
tr|Q7XTS7|Q7XTS7_ORYSJ OSJNBa0008M17.6 protein (Putative unchara... 69 3e-10
tr|Q0JA58|Q0JA58_ORYSJ Os04g0614800 protein OS=Oryza sativa subs... 69 3e-10
tr|Q01K09|Q01K09_ORYSA OSIGBa0126B18.9 protein OS=Oryza sativa G... 69 3e-10
tr|B4XT64|B4XT64_ORYSJ Narrow leaf 1 OS=Oryza sativa subsp. japo... 69 3e-10
tr|A2XXI5|A2XXI5_ORYSI Putative uncharacterized protein OS=Oryza... 69 3e-10
tr|Q9FL94|Q9FL94_ARATH Gb|AAC61821.1 OS=Arabidopsis thaliana GN=... 59 2e-07
tr|Q8L622|Q8L622_ARATH Putative uncharacterized protein At5g4503... 59 2e-07
tr|Q9LDF8|Q9LDF8_ARATH Gb|AAF35944.1 (At3g12950) OS=Arabidopsis ... 54 1e-05
tr|A9TZJ8|A9TZJ8_PHYPA Predicted protein (Fragment) OS=Physcomit... 46 0.002
tr|A9RTD9|A9RTD9_PHYPA Predicted protein (Fragment) OS=Physcomit... 41 0.066
tr|A9RD99|A9RD99_PHYPA Predicted protein (Fragment) OS=Physcomit... 40 0.11
tr|A6TSN2|A6TSN2_ALKMQ Beta-lactamase domain protein OS=Alkaliph... 36 2.8
tr|Q89DR2|Q89DR2_BRAJA Carbamoylphosphate synthase large subunit... 35 3.6
tr|A8J0K4|A8J0K4_CHLRE Predicted protein OS=Chlamydomonas reinha... 35 4.7
tr|Q54YG2|Q54YG2_DICDI Extracellular matrix protein ST430 OS=Dic... 35 6.2

>tr|B8LKZ8|B8LKZ8_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 683

Score = 81.3 bits (199), Expect = 6e-14
Identities = 53/109 (48%), Positives = 68/109 (62%)
Frame = +2

Query: 380 YSGGCCSSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAALTNQCSESSAAYFLWPP 559
YSG S SEES LD E ++ TG P + P + A+ Q SESSAA F WPP
Sbjct: 86 YSG----SMQSEESALDREQTV-TGNSGRHPRSDSPPLQAFAS-GGQHSESSAACFRWPP 139

Query: 560 VYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
R +G+A ERA YF G+QK V+ ++E + G QA+TLLDLMTIRA++
Sbjct: 140 SNRLNGTAEERAAYFGGVQKEVDSETLEHLPSGHQATTLLDLMTIRAFH 188


>tr|Q6Z9X3|Q6Z9X3_ORYSJ Os08g0407200 protein OS=Oryza sativa subsp.
japonica GN=P0453D01.16-1 PE=2 SV=1
Length = 590

Score = 78.2 bits (191), Expect = 5e-13
Identities = 52/103 (50%), Positives = 59/103 (57%), Gaps = 1/103 (0%)
Frame = +2

Query: 401 SPVSEESTLDCEISLCTGGRNHQPIIECAPSP-SSAALTNQCSESSAAYFLWPPVYRPHG 577
S SE S LD E + C NH C PSP A Q SESSAAYF WP HG
Sbjct: 13 SSQSEGSALDMERNGC----NHN----CCPSPLQPIASGGQHSESSAAYFSWPTSTLMHG 64

Query: 578 SAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
SA RANYF LQK V G + R+ G +A+TLLDLM IRA++
Sbjct: 65 SAEGRANYFGNLQKGVLPGHLGRLPTGQRATTLLDLMIIRAFH 107


>tr|A7P2T6|A7P2T6_VITVI Chromosome chr1 scaffold_5, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00030177001
PE=4 SV=1
Length = 596

Score = 78.2 bits (191), Expect = 5e-13
Identities = 48/100 (48%), Positives = 62/100 (62%), Gaps = 1/100 (1%)
Frame = +2

Query: 410 SEESTLDCEISLCTGGRNHQPIIECAPSPSSA-ALTNQCSESSAAYFLWPPVYRPHGSAG 586
SEES LD E + C NH + +P P A A Q SES+AAYF WP R + +A
Sbjct: 18 SEESALDLERNYC----NHPNLPSPSPPPLQAFASGGQLSESNAAYFSWPTSSRLNDAAE 73

Query: 587 ERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
+RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 74 DRANYFGNLQKGVLPETLGRLPTGQQATTLLELMTIRAFH 113


>tr|A5BPH0|A5BPH0_VITVI Putative uncharacterized protein OS=Vitis
vinifera GN=VITISV_008456 PE=4 SV=1
Length = 1092

Score = 78.2 bits (191), Expect = 5e-13
Identities = 48/100 (48%), Positives = 62/100 (62%), Gaps = 1/100 (1%)
Frame = +2

Query: 410 SEESTLDCEISLCTGGRNHQPIIECAPSPSSA-ALTNQCSESSAAYFLWPPVYRPHGSAG 586
SEES LD E + C NH + +P P A A Q SES+AAYF WP R + +A
Sbjct: 452 SEESALDLERNYC----NHPNLPSPSPPPLQAFASGGQLSESNAAYFSWPTSSRLNDAAE 507

Query: 587 ERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
+RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 508 DRANYFGNLQKGVLPETLGRLPTGQQATTLLELMTIRAFH 547


>tr|A2YV45|A2YV45_ORYSI Putative uncharacterized protein OS=Oryza
sativa subsp. indica GN=OsI_29197 PE=4 SV=1
Length = 590

Score = 78.2 bits (191), Expect = 5e-13
Identities = 52/103 (50%), Positives = 59/103 (57%), Gaps = 1/103 (0%)
Frame = +2

Query: 401 SPVSEESTLDCEISLCTGGRNHQPIIECAPSP-SSAALTNQCSESSAAYFLWPPVYRPHG 577
S SE S LD E + C NH C PSP A Q SESSAAYF WP HG
Sbjct: 13 SSQSEGSALDMERNGC----NHN----CCPSPLQPIASGGQHSESSAAYFSWPTSTLMHG 64

Query: 578 SAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
SA RANYF LQK V G + R+ G +A+TLLDLM IRA++
Sbjct: 65 SAEGRANYFGNLQKGVLPGHLGRLPTGQRATTLLDLMIIRAFH 107


>tr|A7PWT6|A7PWT6_VITVI Chromosome chr19 scaffold_35, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00025767001
PE=4 SV=1
Length = 603

Score = 73.2 bits (178), Expect = 2e-11
Identities = 48/116 (41%), Positives = 67/116 (57%)
Frame = +2

Query: 359 MNGSKIFYSGGCCSSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAALTNQCSESSA 538
M+ +K+ C S +SEES + E + C +H P P+ A Q SES+A
Sbjct: 1 MDQTKLNLRLRCSGSTLSEESAPNQERNCCC--HSHLPS-SSLPTLQPFASAGQHSESNA 57

Query: 539 AYFLWPPVYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
AYF WP R + +A ERANYF LQKAV + + KG QA++LL++MTIRA++
Sbjct: 58 AYFSWPTSSRLNDAAEERANYFSNLQKAVLSETPGPLPKGQQATSLLEVMTIRAFH 113


>tr|A2Q289|A2Q289_MEDTR Peptidase, trypsin-like serine and cysteine
proteases OS=Medicago truncatula
GN=MtrDRAFT_AC149576g3v2 PE=4 SV=1
Length = 597

Score = 70.9 bits (172), Expect = 8e-11
Identities = 48/117 (41%), Positives = 67/117 (57%), Gaps = 1/117 (0%)
Frame = +2

Query: 359 MNGSKIFYSGGCCSSPVSEESTLDCEISLCTGGRNHQPIIECAP-SPSSAALTNQCSESS 535
MN +++ S S SEES LD E RN+ +P + A+ Q SE +
Sbjct: 1 MNRNRLGLSAHHSGSTQSEESALDLE-------RNYYGHPSSSPLHMQTFAVGVQHSEGN 53

Query: 536 AAYFLWPPVYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
AAYF WP + R + +A +RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 54 AAYFSWPTLNRWNDAAEDRANYFGNLQKGVLPETLGRLPSGQQATTLLELMTIRAFH 110


>tr|A2Q138|A2Q138_MEDTR Peptidase, trypsin-like serine and cysteine
proteases (Fragment) OS=Medicago truncatula
GN=MtrDRAFT_AC147481g36v2 PE=4 SV=1
Length = 546

Score = 70.9 bits (172), Expect = 8e-11
Identities = 48/117 (41%), Positives = 67/117 (57%), Gaps = 1/117 (0%)
Frame = +2

Query: 359 MNGSKIFYSGGCCSSPVSEESTLDCEISLCTGGRNHQPIIECAP-SPSSAALTNQCSESS 535
MN +++ S S SEES LD E RN+ +P + A+ Q SE +
Sbjct: 1 MNRNRLGLSAHHSGSTQSEESALDLE-------RNYYGHPSSSPLHMQTFAVGVQHSEGN 53

Query: 536 AAYFLWPPVYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
AAYF WP + R + +A +RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 54 AAYFSWPTLNRWNDAAEDRANYFGNLQKGVLPETLGRLPSGQQATTLLELMTIRAFH 110


>tr|Q93YT5|Q93YT5_ARATH Putative uncharacterized protein At2g35155
OS=Arabidopsis thaliana GN=At2g35155 PE=2 SV=1
Length = 579

Score = 69.3 bits (168), Expect = 2e-10
Identities = 46/109 (42%), Positives = 62/109 (56%), Gaps = 6/109 (5%)
Frame = +2

Query: 398 SSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAA------LTNQCSESSAAYFLWPP 559
+S SE+S LD E RNH PS SS + L Q +ES+A YF WP
Sbjct: 15 ASSESEDSALDLE-------RNHHCNHLSLPSSSSPSPLQPFTLNIQHAESNAPYFSWPT 67

Query: 560 VYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
+ R + + +RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 68 LSRLNDTVEDRANYFGNLQKGVLPETVGRLPSGQQATTLLELMTIRAFH 116


>tr|Q8S8Q2|Q8S8Q2_ARATH Expressed protein (Putative uncharacterized
protein At2g35155) OS=Arabidopsis thaliana GN=At2g35155
PE=2 SV=1
Length = 579

Score = 69.3 bits (168), Expect = 2e-10
Identities = 46/109 (42%), Positives = 62/109 (56%), Gaps = 6/109 (5%)
Frame = +2

Query: 398 SSPVSEESTLDCEISLCTGGRNHQPIIECAPSPSSAA------LTNQCSESSAAYFLWPP 559
+S SE+S LD E RNH PS SS + L Q +ES+A YF WP
Sbjct: 15 ASSESEDSALDLE-------RNHHCNHLSLPSSSSPSPLQPFTLNIQHAESNAPYFSWPT 67

Query: 560 VYRPHGSAGERANYFQGLQKAVEFGSIERILKGPQASTLLDLMTIRAYN 706
+ R + + +RANYF LQK V ++ R+ G QA+TLL+LMTIRA++
Sbjct: 68 LSRLNDTVEDRANYFGNLQKGVLPETVGRLPSGQQATTLLELMTIRAFH 116