DK946014
Clone id YMU02A01NGRL0011_C22
Library
Length 334
Definition Adiantum capillus-veneris mRNA. clone: YMU02A01NGRL0011_C22. 5' end sequence.
Accession
Tissue type young leaves
Developmental stage sporophyte
Contig ID
Sequence
GAGTGATTGAGGCATCATCAAAGGGGAAAAGCAACTATTATGAGGCAAAAGTCTTGGTAC
AAGCTTGGAGGAACATCAAGTCTTTGGAGAGTTTCAAACAGATGAAGAAGTCTGGATCTA
CGGATGTCGAAACAAATGTATAAATTAGTTTTAAGCATGATGGAGCAATCCCAATGTCTA
AGAATTTTCGAAGCATGAAGGAGCAACCCCGGTCATTGCACATATGACTGTTGTAATCTC
AACCTAGGTTGATTGTTGTCTTGTTAAGGTACATTTTCCCGGTTGTAATCTATGCATGGT
ACATGTCACAAAAAAAAAAAAAAAAAAAAAAAAA
■■Homology search results ■■ -
sp_hit_id Q945Q1
Definition sp|Q945Q1|CYT1_ARATH Cysteine proteinase inhibitor 1 OS=Arabidopsis thaliana
Align length 33
Score (bit) 35.8
E-value 0.068
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK946014|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0011_C22, 5'
(309 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q945Q1|CYT1_ARATH Cysteine proteinase inhibitor 1 OS=Arabidop... 36 0.068
sp|Q10992|CYTA_HELAN Cysteine proteinase inhibitor A OS=Helianth... 35 0.089
sp|Q8LC76|CYT7_ARATH Cysteine proteinase inhibitor 7 OS=Arabidop... 34 0.20
sp|Q9LEI7|CYT1_HORVU Cysteine proteinase inhibitor OS=Hordeum vu... 34 0.26
sp|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor OS=Vigna ungu... 33 0.44
sp|Q8H0X6|CYT6_ARATH Cysteine proteinase inhibitor 6 OS=Arabidop... 33 0.58
sp|P37842|CYTM_SOLTU Multicystatin OS=Solanum tuberosum PE=2 SV=1 32 0.99
sp|Q41906|CYT3_ARATH Cysteine proteinase inhibitor 3 OS=Arabidop... 32 0.99
sp|P20907|CYT2_ORYSJ Cysteine proteinase inhibitor 2 OS=Oryza sa... 32 0.99
sp|P31726|CYT1_MAIZE Cystatin-1 OS=Zea mays GN=RAMDAZC7 PE=2 SV=1 31 2.2
sp|Q03196|CYT_SOLTU Cysteine proteinase inhibitor (Fragment) OS=... 30 2.9
sp|Q84WT8|CYT4_ARATH Cysteine proteinase inhibitor 4 OS=Arabidop... 30 3.8
sp|Q5EB47|LN28A_XENTR Lin-28 homolog A OS=Xenopus tropicalis GN=... 29 6.4
sp|P04755|ACH3_DROME Acetylcholine receptor subunit beta-like 1 ... 29 6.4
sp|Q86VV8|RTTN_HUMAN Rotatin OS=Homo sapiens GN=RTTN PE=2 SV=2 29 8.3
sp|Q22695|CD5R1_CAEEL Cyclin-dependent kinase 5 activator 1 OS=C... 29 8.4

>sp|Q945Q1|CYT1_ARATH Cysteine proteinase inhibitor 1 OS=Arabidopsis
thaliana GN=CYS1 PE=1 SV=2
Length = 101

Score = 35.8 bits (81), Expect = 0.068
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQM 104
+E + + YEAKVL +AW N+K LESF +
Sbjct: 66 VEVADGETNKVYEAKVLEKAWENLKQLESFNHL 98


>sp|Q10992|CYTA_HELAN Cysteine proteinase inhibitor A OS=Helianthus
annuus PE=1 SV=1
Length = 82

Score = 35.4 bits (80), Expect = 0.089
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGS 119
+EA++ G YEAKV V+ W N K L+ FK + + S
Sbjct: 45 LEATNGGVKKTYEAKVWVKPWENFKELQEFKPVDAATS 82


>sp|Q8LC76|CYT7_ARATH Cysteine proteinase inhibitor 7 OS=Arabidopsis
thaliana GN=CYS7 PE=2 SV=2
Length = 232

Score = 34.3 bits (77), Expect = 0.20
Identities = 19/53 (35%), Positives = 26/53 (49%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDVETNV*ISFKHDG 164
+E G+ YEAKV V+ W N K L+ FK + S + + FK DG
Sbjct: 102 LEVIEAGEKKIYEAKVWVKPWMNFKQLQEFKNIIPSFTISD-----LGFKPDG 149


>sp|Q9LEI7|CYT1_HORVU Cysteine proteinase inhibitor OS=Hordeum
vulgare GN=ICY PE=1 SV=1
Length = 107

Score = 33.9 bits (76), Expect = 0.26
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
IE G YEAKV +AW N K L+ FK
Sbjct: 74 IEVKEGGAKKLYEAKVWEKAWENFKQLQEFK 104


>sp|Q06445|CYTI_VIGUN Cysteine proteinase inhibitor OS=Vigna
unguiculata PE=3 SV=1
Length = 97

Score = 33.1 bits (74), Expect = 0.44
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQM 104
+EA G+ YEAKV + W N K L+ FK +
Sbjct: 60 LEAKDGGQKKVYEAKVWEKPWLNFKELQEFKHV 92


>sp|Q8H0X6|CYT6_ARATH Cysteine proteinase inhibitor 6 OS=Arabidopsis
thaliana GN=CYS6 PE=1 SV=2
Length = 234

Score = 32.7 bits (73), Expect = 0.58
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
+E G+ YEAKV V+ W N K L+ FK
Sbjct: 93 LEILEAGQKKLYEAKVWVKPWLNFKELQEFK 123


>sp|P37842|CYTM_SOLTU Multicystatin OS=Solanum tuberosum PE=2 SV=1
Length = 756

Score = 32.0 bits (71), Expect = 0.99
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
+ A+ K YE K+LV+ W N K ++ FK
Sbjct: 719 LAATDARKKEIYETKILVKEWENFKEVQEFK 749



Score = 31.6 bits (70), Expect = 1.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +3

Query: 9 EASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
EA+ G YEAK+L++ W ++K + FK
Sbjct: 60 EATEGGNKKEYEAKILLRKWEDLKKVVGFK 89



Score = 30.8 bits (68), Expect = 2.2
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3

Query: 12 ASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
A+ GK YE K+ V+ W N K + FK
Sbjct: 439 ATDGGKKKIYETKIWVKVWENFKKVVEFK 467



Score = 30.8 bits (68), Expect = 2.2
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = +3

Query: 12 ASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
A GK YEAK+ V+ W N K + FK
Sbjct: 533 AIDAGKKKIYEAKIWVKEWENFKKVIEFK 561



Score = 30.4 bits (67), Expect = 2.9
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3

Query: 12 ASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
A+ GK YEAK+ V+ W + K + FK
Sbjct: 627 ATDAGKKKIYEAKIWVKEWEDFKKVVEFK 655



Score = 29.3 bits (64), Expect = 6.4
Identities = 15/40 (37%), Positives = 21/40 (52%), Gaps = 5/40 (12%)
Frame = +3

Query: 24 GKSNYYEAKVLVQAWRNIKSLESFKQM-----KKSGSTDV 128
GK Y+AK+ V+ W + K + FK + K G TDV
Sbjct: 160 GKKKIYKAKIWVKEWEDFKKVVEFKLVGDDIAKLGGITDV 199


>sp|Q41906|CYT3_ARATH Cysteine proteinase inhibitor 3 OS=Arabidopsis
thaliana GN=CYS3 PE=2 SV=2
Length = 125

Score = 32.0 bits (71), Expect = 0.99
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQ 101
+EA ++ +EAKV V+ W N K L+ FK+
Sbjct: 91 LEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKE 122


>sp|P20907|CYT2_ORYSJ Cysteine proteinase inhibitor 2 OS=Oryza
sativa subsp. japonica GN=Os05g0494200 PE=2 SV=3
Length = 151

Score = 32.0 bits (71), Expect = 0.99
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3

Query: 24 GKSNYYEAKVLVQAWRNIKSLESFKQM 104
G + YEAKV +AW N K L+ FK +
Sbjct: 120 GANKLYEAKVWERAWENFKQLQDFKPL 146


>sp|P31726|CYT1_MAIZE Cystatin-1 OS=Zea mays GN=RAMDAZC7 PE=2 SV=1
Length = 135

Score = 30.8 bits (68), Expect = 2.2
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGS 119
IE YEAKV + W N K L+ FK +++ S
Sbjct: 97 IEVKDGEVKKLYEAKVWEKPWENFKQLQEFKPVEEGAS 134


tr_hit_id A9SCX0
Definition tr|A9SCX0|A9SCX0_PHYPA Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens
Align length 46
Score (bit) 41.6
E-value 0.02
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= DK946014|Adiantum capillus-veneris mRNA, clone:
YMU02A01NGRL0011_C22, 5'
(309 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9SCX0|A9SCX0_PHYPA Predicted protein (Fragment) OS=Physcomit... 42 0.020
tr|Q8VX72|Q8VX72_RUMOB Putative cysteine proteinase inhibitor OS... 41 0.034
tr|A9PCX4|A9PCX4_POPTR Putative uncharacterized protein OS=Popul... 39 0.13
tr|O24462|O24462_PYRCO Cysteine protease inhibitor OS=Pyrus comm... 39 0.17
tr|A0FK05|A0FK05_POPTO Cysteine protease inhibitor OS=Populus to... 39 0.17
tr|A0FK03|A0FK03_POPTO Cysteine protease inhibitor OS=Populus to... 39 0.17
tr|Q0GPA4|Q0GPA4_AMAHP Cystatin OS=Amaranthus hypochondriacus GN... 38 0.22
tr|A9TIJ1|A9TIJ1_PHYPA Predicted protein OS=Physcomitrella paten... 38 0.22
tr|A7PTC9|A7PTC9_VITVI Chromosome chr8 scaffold_29, whole genome... 38 0.22
tr|Q9SE08|Q9SE08_SOLLC Cystatin (Fragment) OS=Solanum lycopersic... 38 0.29
tr|Q7M224|Q7M224_PERAE Cystatin OS=Persea americana PE=1 SV=1 38 0.29
tr|A5ANX3|A5ANX3_VITVI Putative uncharacterized protein OS=Vitis... 38 0.29
tr|O04720|O04720_SOYBN Cysteine proteinase inhibitor OS=Glycine ... 37 0.38
tr|A9P9T5|A9P9T5_POPTR Putative uncharacterized protein OS=Popul... 37 0.38
tr|Q43635|Q43635_RICCO Cysteine proteinase inhibitor OS=Ricinus ... 37 0.50
tr|Q8L5J8|Q8L5J8_COLES Cysteine proteinase inhibitor OS=Colocasi... 37 0.65
tr|Q4GZT8|Q4GZT8_FRAAN Fa-CPI1 protein (Cystatin CPI-1) OS=Fraga... 37 0.65
tr|Q5ZFY0|Q5ZFY0_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFX6|Q5ZFX6_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFX4|Q5ZFX4_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFX2|Q5ZFX2_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFW9|Q5ZFW9_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFW8|Q5ZFW8_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFW3|Q5ZFW3_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFW0|Q5ZFW0_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFV7|Q5ZFV7_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFV6|Q5ZFV6_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFV4|Q5ZFV4_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFU7|Q5ZFU7_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85
tr|Q5ZFU3|Q5ZFU3_POPTN Cysteine protease inhibitor OS=Populus tr... 36 0.85

>tr|A9SCX0|A9SCX0_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_127641 PE=4 SV=1
Length = 214

Score = 41.6 bits (96), Expect = 0.020
Identities = 20/46 (43%), Positives = 25/46 (54%)
Frame = +3

Query: 3 VIEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDVETNV 140
VIE GK + YEAKV V+ W N K LE FK ++ T + V
Sbjct: 66 VIEVEESGKLSQYEAKVWVKPWENFKKLEDFKPKEQEVFTSADLGV 111


>tr|Q8VX72|Q8VX72_RUMOB Putative cysteine proteinase inhibitor
OS=Rumex obtusifolius PE=4 SV=1
Length = 99

Score = 40.8 bits (94), Expect = 0.034
Identities = 18/39 (46%), Positives = 24/39 (61%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGST 122
+EA+ GK YEAKV V+ W N K ++ FK + GST
Sbjct: 60 LEATDGGKKKVYEAKVWVKPWMNFKQVQEFKLLGDQGST 98


>tr|A9PCX4|A9PCX4_POPTR Putative uncharacterized protein OS=Populus
trichocarpa PE=2 SV=1
Length = 138

Score = 38.9 bits (89), Expect = 0.13
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDV 128
+EA+ G + YE KV V+ W N K L+ FK ++ S+D+
Sbjct: 92 VEATDAGNNKMYEVKVWVKPWMNFKQLQEFKHVEGGTSSDL 132


>tr|O24462|O24462_PYRCO Cysteine protease inhibitor OS=Pyrus
communis PE=4 SV=1
Length = 95

Score = 38.5 bits (88), Expect = 0.17
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKS 113
IEA+ GK YEAKV V+ W N K ++ FK + S
Sbjct: 60 IEATEGGKKKVYEAKVWVKPWENFKQVQEFKPVSDS 95


>tr|A0FK05|A0FK05_POPTO Cysteine protease inhibitor OS=Populus
tomentosa PE=2 SV=1
Length = 138

Score = 38.5 bits (88), Expect = 0.17
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDV 128
+EA+ G + YE KV V+ W N K L+ FK ++ S+D+
Sbjct: 92 LEATDAGNNKMYEVKVWVKPWMNFKQLQEFKHVEGGTSSDL 132


>tr|A0FK03|A0FK03_POPTO Cysteine protease inhibitor OS=Populus
tomentosa PE=4 SV=1
Length = 138

Score = 38.5 bits (88), Expect = 0.17
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDV 128
+EA+ G + YE KV V+ W N K L+ FK ++ S+D+
Sbjct: 92 LEATDAGNNKMYEVKVWVKPWMNFKQLQEFKHVEGGTSSDL 132


>tr|Q0GPA4|Q0GPA4_AMAHP Cystatin OS=Amaranthus hypochondriacus
GN=CPI PE=2 SV=1
Length = 247

Score = 38.1 bits (87), Expect = 0.22
Identities = 18/38 (47%), Positives = 22/38 (57%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGS 119
IEA GK Y+AKV V+ W N K L+ FK + S S
Sbjct: 101 IEAIDAGKKKLYDAKVWVKPWMNFKELQEFKHTEDSPS 138


>tr|A9TIJ1|A9TIJ1_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_195387 PE=4 SV=1
Length = 209

Score = 38.1 bits (87), Expect = 0.22
Identities = 20/46 (43%), Positives = 25/46 (54%)
Frame = +3

Query: 3 VIEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGSTDVETNV 140
VIE YEAKV V+ W+N K LE FK +K +G T + V
Sbjct: 61 VIEVEEGSSIKLYEAKVWVKPWQNFKKLEEFK-LKDAGVTSADLGV 105


>tr|A7PTC9|A7PTC9_VITVI Chromosome chr8 scaffold_29, whole genome
shotgun sequence OS=Vitis vinifera GN=GSVIVT00023307001
PE=4 SV=1
Length = 201

Score = 38.1 bits (87), Expect = 0.22
Identities = 17/38 (44%), Positives = 23/38 (60%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFKQMKKSGS 119
+EA GK YEAKV V+ W N K L+ FK +++ S
Sbjct: 60 LEAIDAGKKKIYEAKVWVKPWMNFKELQEFKHSQETKS 97


>tr|Q9SE08|Q9SE08_SOLLC Cystatin (Fragment) OS=Solanum lycopersicum
GN=STC PE=2 SV=1
Length = 94

Score = 37.7 bits (86), Expect = 0.29
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = +3

Query: 6 IEASSKGKSNYYEAKVLVQAWRNIKSLESFK 98
+EA+ GK YEAKV V+ W+N K +E FK
Sbjct: 56 LEATEGGKKKAYEAKVWVKPWQNFKQVEDFK 86