BP918729
Clone id YMU001_000116_G12
Library
Length 547
Definition Adiantum capillus-veneris mRNA. clone: YMU001_000116_G12.
Accession
Tissue type prothallium
Developmental stage -
Contig ID -
Sequence
TCCAAATGTGCAAAAATTTCAAAAGTGTACATCGAAGTAGAGAGAATACAGGCCAAAGGC
AGCTCAAAATTTGTAATCTGTCGCCTGAAAACCTTAATTGCGTCTGCTTGCATTGCTTTT
TACTCGGACAGAATATCTTTACCTGCATGAGAGCAATTGGTACTCCAACTCATGATTCCA
TCCGAATTGAGCTCACAATTTTCGCATGGACACAAAAGCTAAAAAGATAAATTTCGCTTC
TATCCATTCCAAACGCACTTGATGGCGTTAACTGTTGCCACAGGGCTGGCTCCTTTGTGA
GATTACAAACAACTAATTTCGGGTTTCTCTTTGCATACAAGTAGAGGAGGTCACCTGAAC
TGACAATTTGAAAATTAAGAGGACCTTTCCCTTTCGCACAGAAATCCTTCATTATGCCAT
ACGGCATTCTAGCAATCTCAATCATCTCCTTGCCGGGCTCCTGAAGCTCCCATATCCTGA
CACTCCTAAAAGTATCTCCTCCATGATACATACCCACTAAAAGAAGCTTGTCTTTGTGCT
CCACGAG
■■Homology search results ■■ -
sp_hit_id Q39090
Definition sp|Q39090|UFO_ARATH Protein UNUSUAL FLORAL ORGANS OS=Arabidopsis thaliana
Align length 102
Score (bit) 42.4
E-value 0.001
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP918729|Adiantum capillus-veneris mRNA, clone:
YMU001_000116_G12.
(547 letters)

Database: uniprot_sprot.fasta
412,525 sequences; 148,809,765 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

sp|Q39090|UFO_ARATH Protein UNUSUAL FLORAL ORGANS OS=Arabidopsis... 42 0.001
sp|Q9M310|FBK77_ARATH F-box/Kelch-repeat protein At3g61590 OS=Ar... 39 0.022
sp|P19239|NCAP_MOPEI Nucleoprotein OS=Mopeia virus GN=N PE=3 SV=1 34 0.53
sp|Q9LFV5|FK111_ARATH F-box/Kelch-repeat protein At5g15710 OS=Ar... 33 1.2
sp|Q2A068|NCAP_MOBVC Nucleoprotein OS=Mobala virus (isolate Rat/... 31 4.5
sp|O08918|CCNG2_MOUSE Cyclin-G2 OS=Mus musculus GN=Ccng2 PE=2 SV=3 30 5.8
sp|Q8BD31|NCAP_TAMVU Nucleoprotein OS=Tamiami virus (isolate Rat... 30 7.7

>sp|Q39090|UFO_ARATH Protein UNUSUAL FLORAL ORGANS OS=Arabidopsis
thaliana GN=UFO PE=1 SV=2
Length = 442

Score = 42.4 bits (98), Expect = 0.001
Identities = 26/102 (25%), Positives = 50/102 (49%), Gaps = 2/102 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
L+E K +L+LV + +S+R+W LQ+ +EI RMP + F A+ +G
Sbjct: 301 LLESKGRLILVAAVEKSKLNVPKSLRLWSLQQDNATWVEIERMPQPLYTQFAAE-EGGKG 359

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFG 248
F+ V + + + + + ++ ++ ++ LW P S G
Sbjct: 360 FECVGNQEFVMIVLRGTSLQLLFDIVRKSWLWVPPCPYSGSG 401


>sp|Q9M310|FBK77_ARATH F-box/Kelch-repeat protein At3g61590
OS=Arabidopsis thaliana GN=At3g61590 PE=2 SV=1
Length = 411

Score = 38.5 bits (88), Expect = 0.022
Identities = 27/100 (27%), Positives = 51/100 (51%), Gaps = 5/100 (5%)
Frame = -1

Query: 547 LVEHKDKLLLVG---MYHGGDTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPL 377
L+ +++L++VG + + + + IW L+ GKE +E+A+MP + F G
Sbjct: 296 LMNLRERLVIVGGIGKHDRPEVIKGIGIWVLK--GKEWVEMAKMPQRFFQGF-----GEF 348

Query: 376 NFQIVSSG--DLLYLYAKRNPKLVVCNLTKEPALWQQLTP 263
+ SSG DL+Y+ + +P L+ ++ + W Q P
Sbjct: 349 DEVFASSGTDDLVYIQSYGSPALLTFDMNLKYWRWSQKCP 388


>sp|P19239|NCAP_MOPEI Nucleoprotein OS=Mopeia virus GN=N PE=3 SV=1
Length = 570

Score = 33.9 bits (76), Expect = 0.53
Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 5/83 (6%)
Frame = -1

Query: 547 LVEHKDKLLLVGM---YHGGDTFRS--VRIWELQEPGKEMIEIARMPYGIMKDFCAKGKG 383
L + + L +VGM + G+T VR+W+++ P + MP + C +G+
Sbjct: 134 LEQRRVLLQMVGMGGGFRAGNTLGDGIVRVWDVRNPELLNNQFGTMPSLTIACMCKQGQA 193

Query: 382 PLNFQIVSSGDLLYLYAKRNPKL 314
LN I S DL +Y + P +
Sbjct: 194 DLNDVIQSLSDLGLVYTAKYPNM 216


>sp|Q9LFV5|FK111_ARATH F-box/Kelch-repeat protein At5g15710
OS=Arabidopsis thaliana GN=At5g15710 PE=2 SV=1
Length = 448

Score = 32.7 bits (73), Expect = 1.2
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = -1

Query: 529 KLLLVGMYHGGDTFRSVRIWELQEPGKEMIEIARMP 422
+L LVG T +S+RIWEL +EI+RMP
Sbjct: 342 RLFLVGRIGLYSTLQSMRIWELDHTKVSWVEISRMP 377


>sp|Q2A068|NCAP_MOBVC Nucleoprotein OS=Mobala virus (isolate
Rat/Central African Republic/Acar 3080/1983) GN=N PE=3
SV=1
Length = 568

Score = 30.8 bits (68), Expect = 4.5
Identities = 24/84 (28%), Positives = 38/84 (45%), Gaps = 6/84 (7%)
Frame = -1

Query: 547 LVEHKDKLLLVGMY------HGGDTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGK 386
L + K L LVGM GGD VR+W+++ P + MP + C +G+
Sbjct: 134 LDQRKILLQLVGMGGSRVPPRGGDGI--VRVWDVRNPDLLNNQFGTMPSLTLACLCKQGQ 191

Query: 385 GPLNFQIVSSGDLLYLYAKRNPKL 314
L+ + + DL +Y + P L
Sbjct: 192 EDLSDVVKALTDLGLVYTAKYPNL 215


>sp|O08918|CCNG2_MOUSE Cyclin-G2 OS=Mus musculus GN=Ccng2 PE=2 SV=3
Length = 344

Score = 30.4 bits (67), Expect = 5.8
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3

Query: 45 NTGQRQLKICNLSPENLNCVCLHCFLLGQNIFTCMRAIGTPTHDSIRI 188
N R L + + P++L+C+ + CFLL + + PTHD IRI
Sbjct: 82 NILDRFLALMKVKPKHLSCIGVCCFLLAARLAEEEGDV-PPTHDVIRI 128


>sp|Q8BD31|NCAP_TAMVU Nucleoprotein OS=Tamiami virus (isolate
Rat/United States/W 10777/1964) GN=N PE=3 SV=1
Length = 562

Score = 30.0 bits (66), Expect = 7.7
Identities = 22/80 (27%), Positives = 34/80 (42%), Gaps = 2/80 (2%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTFRS--VRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
L + L LVG+ G R V+IW+++EP + + P + +G LN
Sbjct: 134 LTRRSEILQLVGIQRPGLNRRGGVVKIWDIKEPALLINQFGSTPAVTISCMAEQGGETLN 193

Query: 373 FQIVSSGDLLYLYAKRNPKL 314
+ DL LY + P L
Sbjct: 194 DVVQGLTDLGLLYTAKFPNL 213


tr_hit_id A9SDY4
Definition tr|A9SDY4|A9SDY4_PHYPA Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens
Align length 73
Score (bit) 69.7
E-value 9.0e-11
Report
BLASTX 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.

Query= BP918729|Adiantum capillus-veneris mRNA, clone:
YMU001_000116_G12.
(547 letters)

Database: uniprot_trembl.fasta
7,341,751 sequences; 2,391,615,440 total letters

Searching..................................................done



Score E
Sequences producing significant alignments: (bits) Value

tr|A9SDY4|A9SDY4_PHYPA Predicted protein (Fragment) OS=Physcomit... 70 9e-11
tr|Q6QVX5|Q6QVX5_SYRVU Fimbriata (Fragment) OS=Syringa vulgaris ... 51 3e-05
tr|B8LMU8|B8LMU8_PICSI Putative uncharacterized protein OS=Picea... 51 3e-05
tr|Q84UE8|Q84UE8_LOTJA Proliferating floral organs protein (UFO-... 50 1e-04
tr|A9TDG5|A9TDG5_PHYPA Predicted protein OS=Physcomitrella paten... 49 1e-04
tr|Q84JN4|Q84JN4_PEA Stp-h2 (Stp-h3) OS=Pisum sativum PE=4 SV=1 49 2e-04
tr|A9TXI0|A9TXI0_PHYPA Predicted protein OS=Physcomitrella paten... 49 2e-04
tr|A9U4F8|A9U4F8_PHYPA Predicted protein OS=Physcomitrella paten... 49 2e-04
tr|Q5BMD2|Q5BMD2_MEDTR Stamina pistilloidia (Fragment) OS=Medica... 47 5e-04
tr|Q84L18|Q84L18_PEA Stp-h4 OS=Pisum sativum PE=4 SV=1 47 7e-04
tr|A9RPC5|A9RPC5_PHYPA Predicted protein OS=Physcomitrella paten... 47 7e-04
tr|A9TZQ0|A9TZQ0_PHYPA Predicted protein OS=Physcomitrella paten... 47 9e-04
tr|Q9ZTZ0|Q9ZTZ0_PEA Stamina pistilloidia OS=Pisum sativum GN=St... 46 0.001
tr|A9RIS8|A9RIS8_PHYPA Predicted protein OS=Physcomitrella paten... 45 0.002
tr|Q6QVX4|Q6QVX4_9LAMI Fimbriata (Fragment) OS=Chelone glabra GN... 43 0.009
tr|Q6QVX3|Q6QVX3_VEROI Fimbriata (Fragment) OS=Verbena officinal... 43 0.012
tr|Q6QVX0|Q6QVX0_PAUTO Fimbriata (Fragment) OS=Paulownia tomento... 43 0.012
tr|A7P2M5|A7P2M5_VITVI Chromosome chr1 scaffold_5, whole genome ... 43 0.012
tr|A5BDR7|A5BDR7_VITVI Putative uncharacterized protein OS=Vitis... 43 0.012
tr|B6CNU4|B6CNU4_PETHY DOUBLE TOP OS=Petunia hybrida GN=DOT PE=2... 42 0.016
tr|A9SK59|A9SK59_PHYPA Predicted protein (Fragment) OS=Physcomit... 42 0.021
tr|Q2WBM6|Q2WBM6_9LAMI Fimbriata protein OS=Misopates orontium G... 42 0.028
tr|B6E0Z1|B6E0Z1_CAPAN ANANTHA OS=Capsicum annuum GN=AN PE=2 SV=1 42 0.028
tr|A7PZQ6|A7PZQ6_VITVI Chromosome chr15 scaffold_40, whole genom... 41 0.047
tr|A9TB91|A9TB91_PHYPA Predicted protein OS=Physcomitrella paten... 40 0.062
tr|A9T141|A9T141_PHYPA Predicted protein OS=Physcomitrella paten... 40 0.080
tr|Q41223|Q41223_ANTMA Fim protein OS=Antirrhinum majus GN=fim P... 39 0.14
tr|B6E0Z0|B6E0Z0_SOLLC ANANTHA (Fragment) OS=Solanum lycopersicu... 39 0.14
tr|B6CNU5|B6CNU5_SOLLC DOUBLE TOP OS=Solanum lycopersicum GN=DOT... 39 0.14
tr|B6D6T5|B6D6T5_SOLPI F-box DOT OS=Solanum pimpinellifolium PE=... 39 0.18

>tr|A9SDY4|A9SDY4_PHYPA Predicted protein (Fragment)
OS=Physcomitrella patens subsp. patens
GN=PHYPADRAFT_44304 PE=4 SV=1
Length = 300

Score = 69.7 bits (169), Expect = 9e-11
Identities = 35/73 (47%), Positives = 46/73 (63%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLNFQ 368
LVEH +LLL+G TF VRIWELQE + +E+ MP + K+F KG+ +FQ
Sbjct: 228 LVEHFGRLLLIGAVRIDQTFEGVRIWELQESTAKWVEVETMPERLFKEFYRKGRMFYSFQ 287

Query: 367 IVSSGDLLYLYAK 329
V SG+LLYL+ K
Sbjct: 288 CVGSGNLLYLHVK 300


>tr|Q6QVX5|Q6QVX5_SYRVU Fimbriata (Fragment) OS=Syringa vulgaris
GN=FIM PE=2 SV=1
Length = 331

Score = 51.2 bits (121), Expect = 3e-05
Identities = 30/103 (29%), Positives = 56/103 (54%), Gaps = 2/103 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
LVE + KL+L+ +T +S+R+W LQE G +EI RMP + F ++ +G
Sbjct: 191 LVESRGKLILIAAVEKSKLNTPKSLRLWALQECGTMWVEIERMPQQLYIQF-SEVEGGQG 249

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGM 245
F V+ G+ + + +++ K ++ N +++ LW P + G+
Sbjct: 250 FNCVAHGEFVVILIQKSEKALLFNFSRKKWLWIPTCPYNVNGV 292


>tr|B8LMU8|B8LMU8_PICSI Putative uncharacterized protein OS=Picea
sitchensis PE=2 SV=1
Length = 416

Score = 51.2 bits (121), Expect = 3e-05
Identities = 38/128 (29%), Positives = 58/128 (45%), Gaps = 11/128 (8%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTFRS-----------VRIWELQEPGKEMIEIARMPYGIMKDF 401
LVE + +LL++G D + IWEL KE +EI R+P I +DF
Sbjct: 288 LVECRGRLLMIGRLSNMDQISPAADQTESNIPRIIIWELDVRHKEWVEIVRVPTEICRDF 347

Query: 400 CAKGKGPLNFQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMDRSEIYLF 221
+ F G+ +Y+ RNP ++V +L K WQ L PS F R + +L
Sbjct: 348 SVPLEIYAPFVCSGLGNHIYITTHRNPNVLVYDLWKN--TWQWL-PSDPFFPRRRDFHLL 404

Query: 220 SFCVHAKI 197
F + ++
Sbjct: 405 GFALEPRL 412


>tr|Q84UE8|Q84UE8_LOTJA Proliferating floral organs protein
(UFO-like protein) OS=Lotus japonicus GN=Pfo PE=2 SV=1
Length = 449

Score = 49.7 bits (117), Expect = 1e-04
Identities = 34/113 (30%), Positives = 58/113 (51%), Gaps = 2/113 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
LVE ++KL+LV + RS+R+W LQ G +E RMP + F A+ +G
Sbjct: 316 LVECREKLMLVAAVEKSKLNVPRSLRVWTLQACGTMWVESERMPQQLYIQF-AEIEGGNG 374

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMDRSEIYLFSF 215
F+ V G+ + + K K ++ +L ++ WQ + P G D E++ F++
Sbjct: 375 FECVGHGEFVVIMIKGTDKALLYDLVRK--RWQWIPPCPYAGYDGFELHGFAY 425


>tr|A9TDG5|A9TDG5_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_91322 PE=4 SV=1
Length = 393

Score = 49.3 bits (116), Expect = 1e-04
Identities = 25/82 (30%), Positives = 46/82 (56%), Gaps = 2/82 (2%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTF--RSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
+VE +++LL+VG + F + +WELQ P ++ + + MP G+ +D L+
Sbjct: 276 VVECQERLLMVGGFGRRPHFVTEGICVWELQPPTRDWVVVQNMPEGLFRDL-LNNSSLLS 334

Query: 373 FQIVSSGDLLYLYAKRNPKLVV 308
F V GD ++L ++ P+L+V
Sbjct: 335 FNCVGHGDRIFLSNRKTPRLIV 356


>tr|Q84JN4|Q84JN4_PEA Stp-h2 (Stp-h3) OS=Pisum sativum PE=4 SV=1
Length = 441

Score = 48.9 bits (115), Expect = 2e-04
Identities = 34/113 (30%), Positives = 56/113 (49%), Gaps = 2/113 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
LVE K KLLLV + +S+R+W LQ G +E RMP + F G
Sbjct: 310 LVECKGKLLLVAAVEKSKLNVPKSLRVWSLQGCGSVWVETERMPQQLYVQFADMENGN-G 368

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMDRSEIYLFSF 215
F+ V +G+ + + K + K +V ++ ++ WQ + P G D E++ F++
Sbjct: 369 FECVGNGEFIVIMIKGSDKGLVYDIGRK--RWQWIPPCPYAGYDGFELHGFAY 419


>tr|A9TXI0|A9TXI0_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_172546 PE=4 SV=1
Length = 861

Score = 48.9 bits (115), Expect = 2e-04
Identities = 31/107 (28%), Positives = 57/107 (53%), Gaps = 5/107 (4%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTF---RSVRIWELQEP--GKEMIEIARMPYGIMKDFCAKGKG 383
LVE + LL+VG F +++R+WEL+E G E IE+ MP ++++FC +
Sbjct: 623 LVECQGSLLIVGRISEDSHFAKPKAIRLWELKEKETGGEWIEVVTMPPALLEEFCKEWTR 682

Query: 382 PLNFQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMD 242
P +F+ G ++Y + R ++ +L+++ +WQ L + D
Sbjct: 683 PTHFRCRGLGSVIYFLSSR---ALMYDLSQK--VWQWLPSGPGYHYD 724


>tr|A9U4F8|A9U4F8_PHYPA Predicted protein OS=Physcomitrella patens
subsp. patens GN=PHYPADRAFT_101961 PE=4 SV=1
Length = 395

Score = 48.5 bits (114), Expect = 2e-04
Identities = 26/96 (27%), Positives = 52/96 (54%), Gaps = 3/96 (3%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGGDTFRSVRIWELQE---PGKEMIEIARMPYGIMKDFCAKGKGPL 377
L++ +++L LVG V +WEL++ GK+ +E+ +MP+ + F K +
Sbjct: 274 LIQRRNRLFLVGGAGTERICEHVHMWELKQVDGEGKQWVEVEKMPHEYFQIFF-KERTAT 332

Query: 376 NFQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQL 269
+ + GDL+Y Y + +++VC+ +K+ W+ L
Sbjct: 333 DLKCSGHGDLVYFYKDSHTQVLVCDFSKKQTEWRWL 368


>tr|Q5BMD2|Q5BMD2_MEDTR Stamina pistilloidia (Fragment) OS=Medicago
truncatula GN=Stp PE=2 SV=1
Length = 422

Score = 47.4 bits (111), Expect = 5e-04
Identities = 34/113 (30%), Positives = 56/113 (49%), Gaps = 2/113 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
LVE K KLLLV + +S+R+W LQ G +E RMP + F G
Sbjct: 313 LVECKGKLLLVAAVEKSKLNVPKSLRVWCLQGCGSVWVESERMPQQLYVQFAEMENGN-G 371

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMDRSEIYLFSF 215
F+ V +G+ + + K + K +V ++ ++ WQ + P G D E++ F++
Sbjct: 372 FECVGNGEFIVIMIKGSDKGLVYDIGRK--RWQWIPPCPYAGYDGFELHGFAY 422


>tr|Q84L18|Q84L18_PEA Stp-h4 OS=Pisum sativum PE=4 SV=1
Length = 443

Score = 47.0 bits (110), Expect = 7e-04
Identities = 33/113 (29%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = -1

Query: 547 LVEHKDKLLLVGMYHGG--DTFRSVRIWELQEPGKEMIEIARMPYGIMKDFCAKGKGPLN 374
LVE KLLLV + +S+R+W LQ G +E RMP + F G
Sbjct: 312 LVECNGKLLLVAAVEKSKLNVPKSLRVWSLQGCGSVWVETERMPQQLYVQFADMENGN-G 370

Query: 373 FQIVSSGDLLYLYAKRNPKLVVCNLTKEPALWQQLTPSSAFGMDRSEIYLFSF 215
F+ V +G+ + + K + K +V ++ ++ WQ + P G D E++ F++
Sbjct: 371 FECVGNGEFIVIMIKGSDKGLVYDIGRK--RWQWIPPCPYAGYDGFELHGFAY 421